Carbamyl Phosphate Synthesis in a Land Snail, Strophocheilus oblongus

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Carbamyl phosphate synthesis in a land snail, Strophocheilus oblongus.

A carbamyl phosphate synthetase has been detected and partially characterized in hepatopancreas tissue of the land snail Strophocheitus oblongus. The enzyme is localized in mitochondria, utilizes L-glutamine, and shows an absolute requirement for N-acetyl-L-glutamate. This unique combination of properties distinguishes the snail enzyme from either of the previously described animal carbamyl pho...

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In molluscs, the number of peripheral neurons far exceeds those found in the central nervous system. Although previous studies on the morphology of the peripheral nervous system exist, details of its organization remain unknown. Moreover, the foot of the terrestrial species has been studied less than that of the aquatic species. As this knowledge is essential for our experimental model, the pul...

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Vascular supply of the central nervous system of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata).

The vascularization of the central nervous system of the snail Megalobulimus oblongus was studied by injection of carmine-gelatin solution into the arterial system and using a histochemical technique for the detection of alkaline phosphatase. The central nervous system of M. oblongus is irrigated by the anterior aorta, from which a series of small branches emerge that supply the subesophageal n...

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Enzymatic Synthesis of Acetyl Phosphate and Formyl Phosphate by Carbamyl Phosphate Synthetase.

The utilization of rarbamyl phosphate and acetyl phosphate for synthetic pruposes has been studied in thii laboratory (l-4). Frog liver carbamyl phosphate synthetaee catalyzes the synthesis of adenosine triphosphate from acetyl phosphate and adenosine diphosphate (4); it has also been briefly reported that the enzyme could synthesize ATP from formylP and ADP (5). The reverse reactions, the synt...

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A Kinetic Study of Carbamyl Phosphate Synthetase.

While it is clear from the stoichiometry of the over-all reaction (Reaction 3) that 2 moles of ATP are utilized per mole of carbamyl phosphate formed (l), equilibrium dialysis experiments indicated that only 1 mole of ATP was bound per mole of enzyme in the absence of acetylglutamate (2). In order to gain more information about the nature of ATP binding by carbamyl phosphate synthetase, kinetic...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1970

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)62581-5